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KMID : 0903519910340040406
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1991 Volume.34 No. 4 p.406 ~ p.407
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Metabolism of 14C - Endosulfan in Carp(Cyprinus carpio L .)
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Suh, Yong Tack
Shim, Jae Han/Lee, Kong Bong
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Abstract
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Fish (Cyprinus carpio L.) were treated with LC_(18) concentration, 4.49 ng/㎖ of ^(14)C endosulfan to study the absorption and metabolism. In an in vivo test, endosulfan was motabolized (65∼80%) in the tissues, only the endosulfan ether was identified indicating that it is the main metabolite of detoxification in carp. However, in an in vitro test endosulfan was converted to the endosulfan α-hydroxyether, endosulfan alcohol and endosulfan ether; the largest quantities were present in tissues where the principal metabolites were endosulfan alcohol and endosulfan ether. The maximum absorption time for tissues (head, muschle, and gut) occured after 8 h exposure. However, the maximum distribution time in liver and kidney was 15 min, 3 h respectively. Radioactivity in the tissue decreased rapidly during the 8 h after treatment.
The amount of total recovered ^(14)C-activity in liver, kidney and muscle or fish was about 80∼90% during the 8 h, after application. The non-extractable radioactivity was increased after 8 h exposure (27∼31%). When endosulfan was incubated with the fish liver, kidney and gut preparations, it was metabolized to water solube and organosoluble metabolites. The addition of NADPH resulted in rapid conversion of endosulte to the metabolites in an in vitro test. The rate of metabolism of endosulfan in liver, kidney and gut supplemented with NADPH as a cofactor was higher in the 105,000 g soluble fraction than microsomes.
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